The lab suggests fixation will bring about the dyes to leak through the cells. I feel the assumption is fixation may possibly hurt cell membranes. I did look for a paper which describes fixation making use of methanol or acetic acid, after staining that has a DCFDA spinoff. DCFDA is https://codyzmxyv.worldblogged.com/35130005/examine-this-report-on-2-fdck-hcl