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The CRISPR/Cas9 system is widely used for genome editing in livestock production. although off-target effects can occur. It is the main method to produce genome-edited goats by somatic cell nuclear transfer (SCNT) of CRISPR/Cas9-mediated genome-edited primary goat fetal fibroblast cells (GFFs). Improving the double-strand break (DSB) efficiency of Cas9 in primary cells would improve t... https://www.chiggate.com/3m-speedglas-9002nc-welding-helmet-fashion/

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